Figure 1 | Scientific Reports

Figure 1

From: High-Throughput Video Processing of Heart Rate Responses in Multiple Wild-type Embryonic Zebrafish per Imaging Field

Figure 1

Image processing using FisHRateZ. (a) original image pulled from the 10-s video file. (b) FisHRateZ identifies embryos and removes all other particles (i.e., lint or dust) in wells. (c) The shape of the embryo allows for geometric determination of heart location for ROI placement. c’) inset of heart region of embryo shows: a Feret diameter, which is the longest line that can be drawn lengthwise across the embryo, a circle superimposed over yolk sac, a second line passing through the circle, and a region of interest rectangle (ROI) as described in the Methods and Materials section. (d) ROI boxes are superimposed over the original image and applied to each frame of the video for pixel intensity versus time plotting.

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