Figure 1 | Scientific Reports

Figure 1

From: Reversible senescence of human colon cancer cells after blockage of mitosis/cytokinesis caused by the CNF1 cyclomodulin from Escherichia coli

Figure 1

Endoreplication and polyploidisation in CNF1-treated HCT-116 cells. (a) Clonogenic assay for the determination of IC50 of CNF1 in HCT-116. Data are mean ± SD of three different experiments. The right panel shows representative pictures of colony formation in untreated cells (control, CTR) and cells treated with different concentrations of CNF1. (b) DNA content analysis of HCT-116 cells treated with increasing concentrations of CNF1 for 72 h (upper panel) or 5 nM CNF1 at different time points during 72 h (lower panel). (c) Representative time-lapse images of CNF1-induced endoreplication in HCT-116. Time is indicated in hours and minutes (h:min). Black arrowheads indicate a filopodium-like structure at the cell periphery; Red arrowheads indicate a cytokinesis failure; White arrowheads indicate continuous endoreplication and multinucleation. Bars, 50 μm.

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