Figure 1

Endothelial-specific eGFP expression in embryoid bodies (EBs) derived from PECAM/eGFP ES cells. (A–C) Fluorescence images show eGFP⁺ clusters at day 6 (A) and early vascular network formation in EBs at day 8 (B) and day 10 (C) of suspension culture. Arrow heads indicate eGFP⁺ sprouts. (D–F) In plated EBs distinct sprouts can be observed at day 10 + 4 (D) resulting in a complex vascular network in the center (E) and the periphery (F) of the EB at day 10 + 7. (G–H) Immunofluorescence stainings of vascular networks reveal the co-localization of native eGFP and PECAM expression. Green = native eGFP, red = PECAM, blue = Hoechst; bars = 200 µm (A–E), 100 µm (F), 50 µm (G–I).