Figure 2
From: PECAM/eGFP transgenic mice for monitoring of angiogenesis in health and disease
EGFP expression in PECAM/eGFP embryos at different developmental stages. (A) Fluorescence picture demonstrates bright native eGFP expression in network-like structures of embryos at E9.5. (B) PECAM-DAB whole mount staining of littermates shows a good overlap with eGFP+ structures in (A) confirming PECAM-specific eGFP expression. (C) In cryosections of E9.5 embryos co-localization of native eGFP (green) and PECAM staining (red) was observed. Strong eGFP (green) and PECAM (red) expression were identified in the branchial arch (BA), branchial arch artery (BAA), outflow tract of the heart (OFT), common atrial chamber (CAC), common ventricular chamber (CVC) as well as in intersomitic vessels (ISV). The dotted white line defines the surface of the embryo. (D–H) Sections of embryos at E15.5 reveal a strong eGFP expression in the developing vasculature (D), in particular in the heart (E), the lung (F), the gut (G), and the paw (H). (I–K) Immunofluorescence stainings of heart sections derived from a E15.5 embryo confirms PECAM-specific eGFP expression in blood vessels. Green = native eGFP, brown = PECAM-DAB, red = PECAM (C,J,K), autofluorescence (AF) (D–H), blue = Hoechst; bars = 500 µm (A–C), 100 µm (D), 200 µm (E–H), 50 µm (I–K).
