Figure 5
The effects of NDUFA11 and SDHC silencing on the mitochondrial membrane potential in H9c2 cells. (A) Representative images of TMRM (for ΔΨm) and MitoTracker Green (for mitochondrial content) fluorescence. Scale bar = 50 µm. (B,C), Results of quantitative analysis of the fluorescent intensity. Integrated pixel intensities from TMRM signals were normalized by the area occupied by MitoTracker Green. Whole images were used for calculations. Two clones of siRNAs per each gene have been used to silence NDUFA11 (NDUFA11-1 and NDUFA11-2) and SDHC (SDHC-1 and SDHC-2). In control group (C), the cells were treated with negative control siRNA. 1.0 µM antimycin A (Ant) was used as a positive control to induce the ΔΨm loss. Results are presented as mean ± SE. *P < 0.05, **P < 0.01 vs. control; n = 3 per group.
