Figure 1

Titration of the gB-BiTE antibody construct. (a) Flow cytometric analysis of the amounts of BiTE antibody construct bound to T cells, which were previously activated and expanded by anti-CD3/CD28-antibody coated beads. (b) Normalized levels of IFN-γ secreted by activated or resting T cells, respectively, upon co-culture with non-infected or HCMV-infected HFF (AD169, MOI 5, day 4 p.i.) and an increasing amount of the BiTE antibody construct (mean ± standard deviation; n = 3). (c) Normalized levels of TNF secreted by CD3/CD28-activated T cells upon co-culture with non-infected or HCMV-infected HFF (AD169, MOI 5, day 4 p.i.) and an increasing concentration of the BiTE antibody construct (mean ± standard deviation; n = 3). IFN-γ and TNF levels were normalized for each donor to the value obtained with activated T cells co-cultured with HCMV-infected HFF and 1000 ng/ml BiTE antibody construct.