Figure 2
From: A gB/CD3 bispecific BiTE antibody construct for targeting Human Cytomegalovirus-infected cells
Comparison of the efficiency of redirecting T cells to gB by either the BiTE antibody construct or a CAR. Activated T cells were redirected by either the gB-BiTE antibody construct or a gB-CAR and co-cultured with 293T cells, which were electroporated with increasing amounts of chimeric EpCAM-gB mRNA, as indicated. (a) Expression levels of EpCAM-gB in the target cells 293T 18 hours after electroporation with different amounts of EpCAM-gB mRNA. (b) Expression of the gB-CAR in CD3/CD28-activated primary T cells of one representative experiment. (c) Proportion of degranulating T cells as determined by flow cytometric analysis of cell surface expression of CD107a. (d) Normalized levels of secreted IFN-γ (values obtained with gB-BiTE antibody construct at 10 µg EpCAM-gB mRNA were set to 100%). (c,d) display the mean values ± standard deviation of experiments with three donors.
