Figure 4
From: A gB/CD3 bispecific BiTE antibody construct for targeting Human Cytomegalovirus-infected cells
Triggering of cytotoxic effector functions by the gB-BiTE antibody construct. (a) Activated T cells were co-cultured with different target cells at an E:T ratio of 25:1 for 4 hours. The target cells 293T were either electroporated with mRNA encoding EpCAM-gB or were mock electroporated. HFF were used for experiments on day 4 after infection (AD169, MOI 5), non-infected cells were used as a control. Data of two representative donors are shown. (b) Proportion of degranulating T cells (characterized by CD107a surface expression) upon co-culture with different target cells as indicated (n = 3).
