Figure 5
From: A gB/CD3 bispecific BiTE antibody construct for targeting Human Cytomegalovirus-infected cells
Inhibition of HCMV infection in HFF by supernatants of T cells redirected by the gB-BiTE antibody construct. (a) Levels of IFN-γ and TNF secreted by activated T cells with or without BiTE antibody construct after co-culture with HFF, non-infected or infected with HCMV (AD169, MOI 5, day 4 p.i.), as indicated (n = 3). For the experiments shown in (b), serial dilutions (1:3, 1:10, 1:30) of the supernatants of the co-cultures analysed in (a) were added to HFF throughout and after the infection with HCMV (AD169, MOI 0.25). The proportion of infected HFF was then analysed 4 days later by flow cytometric detection of GFP expression (n = 3; n = 2 with the supernatants from non-infected HFF without BiTE antibody construct). Additionally, control cultures without added supernatants were analysed for the proportion of infected cells one day after infection. The increase of GFPpos cells until day 4 beyond these reference levels on day one indicates the fraction of cells, which were infected by the newly generated HCMV particles.
