Figure 4

Chromatin accessibility analysis of the mesenchymal stem cells (MSCs) with different tissue origins. (a) Principle component analysis using assay for transposase-accessible chromatin using sequencing (ATAC-seq) data from MSCs. Each dot represents the chromatin accessibility profile of a biological replicate. (b) Pearson correlation of ATAC-seq data. (c) Dendrogram from unsupervised clustering using ATAC-seq data. The height indicates the distance between clusters. (d) Clustering using peaks that were dynamically accessible among cells. The variation in chromatin accessibility was assessed by cisTopic. (e) Pathway analysis of the differentially accessible regions using Ingenuity Pathway Analysis (IPA). The peaks in each topic that were differentially accessible among the mesenchymal stem cells (MSCs) were assigned to the nearest genes. The resulting gene list was analysed by IPA. The top five canonical pathways identified are shown.