Figure 4 | Scientific Reports

Figure 4

From: Genome-Wide Transcriptome Landscape of Embryonic Brain-Derived Neural Stem Cells Exposed to Alcohol with Strain-Specific Cross-Examination in BL6 and CD1 Mice

Figure 4

Strain-specific comparison of the Mecp2 expression and DNA methylation in E14.5 embryonic forebrain of BL6 and CD1 mice. (A) Normalized expression of Mecp2 and its two isoforms (Mecp2e1 and Mecp2e2) compared to Gapdh in E14.5 embryonic forebrain. (B) DNA methylation at single CpG dinucleotides at the Mecp2 regulatory regions (R1-to-R6) conducted by Bisulfite pyrosequencing. (C) Average DNA methylation from R1-to-R3 from the promoter (individual regions), or R4-R6 from intron 1 (combined three regions). (D) Correlation coefficient for DNA methylation at single CpG dinucleotides from R1-to-R6 and Mecp2e1 and Mecp2e2 expression. (E) DNA dot blot from extracted DNA at E14.5 embryonic forebrain in CD1 and BL6 mice. N = 3 ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, or ****P < 0.0001.

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