Figure 5 | Scientific Reports

Figure 5

From: Genome-Wide Transcriptome Landscape of Embryonic Brain-Derived Neural Stem Cells Exposed to Alcohol with Strain-Specific Cross-Examination in BL6 and CD1 Mice

Figure 5

Strain-specific comparison of Mecp2/MeCP2, DNA methylation-related genes, and specific markers in neural stem cells of BL6 and CD1 mice. (A,B) Normalized expression of Mecp2 and its two isoforms (Mecp2e1 and Mecp2e2) compared to Gapdh in E14.5 embryonic forebrain, D0 neural stem cells (neurospheres), D1, and D8 after differentiation in CD1 and BL6 mice. (C) MeCP2 expression by IHC in sectioned neurospheres (D0) in CD1 and BL6 mice. (D,H) Similar to (A–B), but for Dnmt1, Dnmt3a, Tet1, Tet2, and Tet3. (I) IHC detection of indicated specific protein markers in CD1 and BL6 sectioned neurospheres (D0). N = 3 ± SEM. *P < 0.05, **P < 0.01, or ***P < 0.001.

Back to article page