Figure 2

Characterisation of serum-derived enriched EV-fractions from scale-up process. (a) Flow cytometry analysis of CD63 and CD81. MFI, Median Fluorescence Intensity. Protein concentration by the Pierce bicinchoninic acid assay (BCA assay) is shown mg/mL. (b) NTA profiles of EV-enriched fractions from size exclusion chromatography (SEC). Concentration is shown in particle/ mL. (c) Electron microscopy. (c) Representative TEM images. Scales in nanometers (nm).