Figure 3
From: Human Herpesvirus 6A and 6B inhibit in vitro angiogenesis by induction of Human Leukocyte Antigen G
HHV-6 effect on HLA-G expression. (A) HUVECs infected with HHV-6A and HHV-6B respectively for 24 and 48 h.p.i. and analyzed for HLA-G expression by flow cytometry. Staining was performed with anti-HLA-G moAb (87 G moAb) (Exbio, Praha, Czech Republic), and isotypic controls (Exbio, Praha, Czech Republic). The analysis was carried out with a FACSCount cytometer and the CellQuest software (Becton Dickinson, San Jose, CA, USA). Results are expressed as MFI (mean fluorescence intensity). The white histogram represents stained cells, the grey histogram represents isotype control stained cells. (B) Percentage of HLA-G positive HUVECs infected with HHV-6A and HHV-6B respectively for 6, 24, 48 and 72 h.p.i.. (C) sHLA-G levels in HUVECs infected with HHV-6A and HHV-6B respectively for 24, 48 and 72 h.p.i.. D) sHLA-G1 and HLA-G5/G6 levels in HUVECs infected with HHV-6A and HHV-6B respectively for 24, 48 and 72 h.p.i.
