Figure 3
From: Plasmodium falciparum gametocyte-infected erythrocytes do not adhere to human primary erythroblasts
Endogenous levels of STEVOR expression do not promote GIE adhesion to erythroid cells. (a,b) RT-qPCR analysis of transcriptional levels for the 35 stevor genes in trophozoites (28 hours post-infection) of NF54 and 3D7/Pf13 lines (a), and of NF54 clones E10F11, E10D4 and F6 (b). Transcription levels were normalized to the transcription level of the arginyltRNA synthetase reference gene (PF3D7_1218600). (c) IFA of permeabilized GIE showing the expression of STEVOR protein (green) in NF54, 3D7/Pf13, E10F11, E10D4 and F6 parasite lines. GIE were stained with a rabbit polyclonal antibody directed against STEVOR PF3D7_0631900 protein followed by anti-rabbit Alexa488-conjugated IgG. Pictures were taken under identical exposure conditions. Bars represent 5 µm. (d) Cell-cell adhesion assays of MACS-purified GIE from NF54 clones or of uninfected erythrocytes (uE) to K562 cells. Error bars denote the standard error of the mean. ns, non-significant differences in adhesion rates. n = number of experiments.
