Figure 5
From: Plasmodium falciparum gametocyte-infected erythrocytes do not adhere to human primary erythroblasts
STEVOR proteins are not exposed at the GIE surface IFA of permeabilized (a) and non-permeabilized (b) immature GIE from the E10F11, 3D7/Pf13 and Stevor-Ty1 (+BSD) parasite lines. GIE were stained with a rabbit polyclonal antibody directed against the N-terminal domain of STEVOR PF3D7_0631900 (green) and with a mouse antibody directed against the cytoplasmic domain of Band 3 ((a), red) or with a mouse anti-Glycophorin A (GPA) (b, red). Pie charts show proportions of GIE positive (green) or negative (grey) for STEVOR staining. 300 cells per condition were examined. Band 3 and GPA staining control permeabilization and presence of the erythrocyte membrane, respectively. Pictures were taken under identical exposure conditions. Bars represent 5 µm.
