Figure 4

Determination of the cytolytic effect of putrescine (A) and cadaverine (B) on HT29 intestinal cells. RTCA cell culture supernatants were collected after 24 h of incubation with the corresponding concentration of putrescine or cadaverine. BA-induced necrosis was monitored as the release of cytosolic lactate dehydrogenase (LDH) into the culture medium. The data represent the percentage of cells lysed by treatment with different concentrations of putrescine or cadaverine. Data represent the means of at least three independent experiments; vertical bars represent standard deviations.