Figure 3

P. vulgaris pin and thrum flower differential gene expression, and GO term enrichment. (a) Genes with significantly higher expression (<0.05 FDR) in thrum (upregulated in thrum) (n = 283) (red), or pin (downregulated in thrum) (n = 118) (black) flowers, with log₂ fold-change in expression in thrum compared to pin shown (FPKM, Fragments Per Kilobase of transcript per Million fragments mapped); genes expressed in only one morph are excluded; (b) Genes expressed in only one morph: log₁₀ change in expression (FPKM + 1) in response to the presence of the S locus in thrum; red = genes upregulated in thrum flowers (expressed in thrum only) (n = 525); black = genes downregulated in thrum flowers (expressed in pin only) (n = 468); (c) Gene Ontology (GO) terms assigned to the 401 significantly differentially expressed genes (a), and their associated GO-term enrichment scores (top 30 over-represented GO terms (False Discovery Rate (FDR) < 0.1) are shown); enrichment score = −log₁₀(uncorrected p-value), with the uncorrected p-value as calculated in GO-term enrichment analysis versus GO term occurrence in P. vulgaris LH_v2 functional annotations.