Figure 4

Primula veris S locus genetic architecture and gene structures. (a) Genomic read depth across the 455 kb P. vulgaris S locus assembly region in 5 kb non-overlapping windows: P. veris thrum (blue), P. vulgaris thrum (red) and pin (black); grey vertical lines above the x-axis represent ambiguous bases (“N”s) in the assembled sequence. The schematic above the graph shows the approximate size and location of P. vulgaris genes²⁷ within this region: thrum-specific S locus genes (red), tandem-repeat CFB loci that flank the 278 kb S locus (yellow), and predicted genes flanking the S locus (black) are indicated. (b) gene structures of P. veris (Pve) orthologues of the five genes identified in the P. vulgaris S locus²⁷. Exons (thick lines) and introns (thin lines; approximate size) are shown; introns >0.5 kb are displayed as 0.5 kb (see Supplementary Fig. S4 for expanded intron size schematic).