Figure 2

(A) Schematic representation of the operons, including the PCA decarboxylase gene and/or subunit genes, constructed in this study. aroY^opt or kpdC^opt, codon-optimized decarboxylase gene derived from K. pneumoniae; kpdB^opt and kpdD^opt, codon-optimized PCA decarboxylase subunit genes derived from K. pneumoniae. kpdB^opt, kpdC^opt and kpdD^opt genes are transcriptionally coupled. RBS represents ribosome binding site. (B) Production of metabolites by C. glutamicum strains harboring gene operons (presented in panel A). Grey, DHS; sky blue, PCA; blue, MA. Error bars represent standard deviations based on duplicate experiments.