Figure 5
From: Corynebacterium Cell Factory Design and Culture Process Optimization for Muconic Acid Biosynthesis
Time course profiles of cell growth, agitation speed, DO concentration, pH, DCW, glucose, organic acids and metabolite production by the STR011 strain in the 50-L fermenter. (A) The feeding medium was sequentially injected at cultivation time periods of 40, 60, 72, 95 and 116 h at a rate of 1.3608 mL/min, 2.0412 mL/min, 2.7216 mL/min, 3.0618 mL/min and 3.402 mL/min. (B) The feeding medium was sequentially injected at cultivation time periods of 37, 53, 76, 90, 104, 138, and 162 h at a rate of 1.3608 mL/min, 2.0034 mL/min, 2.7216 mL/min, 3.402 mL/min, 3.7422 mL/min, 4.2236 mL/min, 3.402 mL/min. During the fed-batch cultivation, 233.1 g/L and 259.8 g/L of glucose was totally added, respectively.
