Table 1 Plasmids and strains used in this study.

pK19mobsacB

Kan^R; vector for allelic exchange in C. glutamicum; (pK18 oriVE.c., sacB, lacZα)

⁵⁶

pK19aroE

pK19mobsacB carrying fragments of aroE gene upstream and downstream

This work

pK19pcaGH

pK19mobsacB carrying fragments of pcaG and pcaH gene upstream and downstream

This work

pK19catB

pK19mobsacB carrying fragments of catB gene upstream and downstream

This work

pMESK101

pSK1Cat modification vector including restriction sites (BamHI, BglII, MunI, NdeI, PstI, SalI, ScaI, SpeI, and XbaI), oriT and terminator

⁵⁷, This work

pMESK102

pMESK101 carrying fragment of P180_ kpdC^opt

This work

pMESK103

pMESK101 carrying fragment of P180_ aroY^opt

This work

pMESK104

pMESK101 carrying fragment of P180_kpdB kpdC kpdD^opt

This work

pMESK105

pMESK101 carrying fragment of P180_aroY kpdB kpdD^opt

This work

pMESK106

pMESK101 carrying fragment of Ptuf_kpdB kpdC kpdD^opt

This work

pMESK107

pMESK101 carrying fragment of Ptuf_aroY kpdB kpdD^opt

This work

pMESK108

pMESK101 carrying fragment of Psod_kpdB kpdC kpdD^opt

This work

pMESK109

pMESK101 carrying fragment of Psod_aroY kpdB kpdD^opt

This work

Strain

Relevant characteristics

Ref.

C. glutamicum 13032

wild-type strain, biotin-auxotrophic

KCTC

STR001

C. glutamicum 13032 ΔaroE

This work

STR002

C. glutamicum STR001 ΔpcaG, ΔpcaH

This work

STR003

C. glutamicum STR002 ΔcatB

This work

STR004

C. glutamicum STR003/pMESK102

This work

STR005

C. glutamicum STR003/pMESK103

This work

STR006

C. glutamicum STR003/pMESK104

This work

STR007

C. glutamicum STR003/pMESK105

This work

STR008

C. glutamicum STR003/pMESK106

This work

STR009

C. glutamicum STR003/pMESK107

This work

STR010

C. glutamicum STR003/pMESK108

This work

STR011

C. glutamicum STR003/pMESK109

This work