Figure 5
From: Quantifying site-specific chromatin mechanics and DNA damage response
MSDs showing decoupled chromatin dynamics induced by DNA damage. (a) U2OS cell treated with bleocin for 2 hours prior to imaging showing the localization patterns exhibited by GFP-53BP1 and RFP-TRF1. (b) MSDs from RFP-TRF1 (n = 18) in U2OS cells treated with bleocin, shown in red, and GFP-Fibrillarin (n = 13) in untreated control U2OS cells, shown in green, showing similar mobility to each other despite global damage induction, but increased mobility in damage loci marked by GFP-53BP1 (n = 12), shown as blue triangles. Error bars are SEM. GFP-53BP1 previously used in Fig. 4b. GFP-Fibrillarin (Control) previously used in Fig. 1c.
