Figure 2
From: Adult hippocampal neurogenesis occurs in the absence of Presenilin 1 and Presenilin 2
Retroviral ablation of presenilins does not affect NPC survival. (a) Schematic of dual retroviral (RV) approach. Two retroviruses (CAG-GFPCre, CAG-RFP) were bilaterally injected into the dentate gyrus (DG) of control (PS1WT;PS2−/−) and vDKO (viral double knockout; PS1fl/fl;PS2−/−). (b) Representative images of virally labeled cells showing GFPCre+ (green), RFP+ (red) and GFPCre+ RFP+ (yellow) double labeled NPCs at 12 and 30 days post infection (dpi). (c) Quantification of the number of GFPCre+ RFP+ virally labeled cells shows both control and vDKO cells decreasing over time (F(1, 20) = 15.9, p = 0.0007). (d) Quantification of survival shows no change in survival ratio ((GFPCre+ RFP+)/total RFP+) at either 12 or 30 dpi. Scale bar, 50 μm. n = 3–6 mice/genotype at 12 dpi, n = 7–8 mice/genotype at 30 dpi. Data are presented as the mean ± SEM.
