Figure 3
From: Adult hippocampal neurogenesis occurs in the absence of Presenilin 1 and Presenilin 2
Virally labeled NPCs can differentiate into mature granule neurons in the absence of presenilins. (a) Representative images of GFPCre+ cells expressing DCX at 12 dpi. Scale bar, 20 μm. (b) Quantification of the proportion of GFPCre+ cells expressing DCX+ shows no difference between control and vDKO mice at 12 dpi (n = 2–3 mice/genotype). (c) Representative images of GFPCre+ cells expressing NeuN+ at 30 dpi. Scale bar, 20 μm. (d) Quantification of the proportion of GFPCre+ cells expressing NeuN shows that nearly all GFPCre+ cells develop into mature granule neurons irrespective of genotype (n = 3–4 mice/genotype). (e) Sample projections of Z-series stacks (left) and dendritic traces (right) illustrating the dendritic complexity of GFPCre+ RFP+ neurons in control and vDKO cells at 30 dpi. Scale bar, 20 μm. (f) Sholl analysis of dendritic complexity shows no difference between control (grey) and vDKO cells lacking presenilins (black). (n = 4 mice/genotype). Data are presented as the mean ± SEM.
