Figure 6
From: Adult hippocampal neurogenesis occurs in the absence of Presenilin 1 and Presenilin 2
Ablating presenilins using nestin-driven inducible transgenic mouse (nDKO) does not alter the number of stem and progenitor cells and their progeny. (a) Schematic of recombination induced by tamoxifen (TAM) in control (NestinCreERT2;R26RYFP; PS1WT;PS2−/−) and nDKO (nestin-driven double knockout; NestinCreERT2;R26RYFP; PS1fl/fl;PS2−/−) mice. (b) Density-scatter plots of FACS-isolated cells from the dentate gyrus of control (left) and nDKO mice (right). (c) qPCR on YFP+ sorted cells show reduced PS1 mRNA in nDKO cells compared to control cells (t(4) = 6.444, p < 0.005), and no PS2 mRNA detected in either cell group. (d) Representative images of YFP+ cells at 12 and 30 days post-TAM in control and nDKO mice. Scale bar, 50 μm. (e) Quantification of the total number of YFP+ cells shows an accumulation of recombined cells at 30 days compared to 12 days post-TAM (F(1,39) = 11.2, p < 0.005), with no differences between the genotypes. (n = 9–13 mice/genotype at 12 day, 10–11 mice/genotype at 30 day). Data are presented as the mean ± SEM.
