Figure 4 | Scientific Reports

Figure 4

From: Abrogated Caveolin-1 expression via histone modification enzyme Setdb2 regulates brain edema in a mouse model of influenza-associated encephalopathy

Figure 4

Caveolin-1 level in brain vascular endothelial cells (BVECs). (a) Flow cytometry was performed using specific antibodies against CD31 and CD45. Sorted CD31+CD45− cells were defined as BVECs. The purity of this group was >98%. (b) Western blot analysis of Caveolin-1 and β-actin from BVECs of the control, Flu, LPS and IAE (Flu + LPS) groups. Analysis was performed 8 h and 24 h after LPS administration. (c) The bands of Caveolin-1 and β–actin were quantified using Image J. Data are shown as the mean ± SEM (8 h control, n = 4; Flu, n = 4; LPS, n = 4; Flu + LPS, n = 4; 24 h control, n = 4; Flu, n = 4; LPS, n = 4; Flu + LPS, n = 4) and are from a representative experiment of two independent experiments. *p < 0.05; **p < 0.01.

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