Figure 4

Antennal odorant receptor differential transcript abundance between sexes and across ages in Aedes aegypti. Heat plots showing the abundance of odorant receptor (Or) transcripts in female (A) and male (B) Ae. aegypti at 1, 3 and 5 days post-emergence (dpe). Genes within each gene family are presented left to right from most to least abundant, as determined by the mean abundance of all three ages for each sex. All Or transcripts are ordered from left to right from the highest to the lowest quartile, except the co-receptor Orco, which is not included in the quartile analysis, and is found at the extreme left. Note differences in scale in (A,B). Genes differentially abundant between 1 dpe and 5 dpe (◗), and genes differentially abundant between both 1 dpe and 3 dpe, and 1 dpe and 5 dpe (•), were identified using the beta-binomial Baggerley’s test and a false discovery rate (FDR)-corrected p-value (P) of <0.05 (n = 6) with a FC ≥2. Scatter plots showing the differential transcript abundance of Ors in the antennae of both sexes at 1 dpe (C), 3 dpe (D) and 5 dpe (E). Transcripts that exhibited significant differences in abundance (Baggerley’s test; P < 0.05) are colour-coded according to their weighted fold change (FC). The expression levels are shown as the mean Log₁₀ (TPM + 1) for all of the six biological replicates for both sexes and each age.