Figure 5

Antennal ionotropic receptor differential transcript abundance between sexes and across ages in Aedes aegypti. Heat plots showing the abundance of ionotropic receptor (Ir) transcripts in female (A) and male (B) Ae. aegypti at 1, 3 and 5 days post-emergence (dpe). Genes within each gene family are presented left to right from most to least abundant as determined by the mean abundance of all three ages for each sex. All Ir transcripts are ordered from left to right from the highest to the lowest quartile, except the co-receptors Ir25a, Ir76b and Ir8a, which are not included in the quartile analysis, and are found at the extreme left. Note differences in scale in (A,B). Scatter plots showing the differential transcript abundance of Irs in the antennae of both sexes at 1 dpe (C), 3 dpe (D) and 5 dpe (E). Transcript that exhibited significant differences in abundance (Baggerley’s test; P < 0.05) are colour-coded according to their weighted fold change (FC). The expression levels are shown as the mean Log10 (TPM + 1) for all of the six biological replicates for both sexes and each age.