Figure 4

Response of CSC primed B16 F0s to stiffness. (A) Immunofluorescence images of α5β1 (green) in patterned B16 F0, non-patterned B16 F0s, and non-patterned B16 F10; nuclei stained with Hoescht (blue). (B) Quantification of fluorescence intensity with ImageJ. (C) Example of dry mass growth rate of cells (D) Average velocity width distribution of cells. N = 3. TCP-tissue culture plastic; PAT - pattern. Error bars indicate ± SEM. Scale bar indicate 50 μm. **p-value < 0.05 (0.034), *p-value < 0.1 (0.058).