Table 1 Primers Used in This Study.
Gene | Primer (5′ to 3′) | Amplification Size (bp) |
|---|---|---|
RT-PCR | ||
Vegf (rat) | For, CACTGGACCCTGGCTTTACT Rev, GACGTCCATGAACTTCACCA | 111 |
Hif1α (rat) | For, TGGTGCTAACAGATGATGGTG Rev, CATGGTCACATGGATGGGTA | 123 |
Gapdh (rat) | For, CTCATGACCACAGTCCATGC Rev, TTCAGCTCTGGGATGACCTT | 155 |
VEGF (human) | For, CCTTGCTGCTCTACCTCCAC Rev, GCAGTAGCTGCGCTGATAGA | 119 |
GAPDH (human) | For, AGGTGAAGGTCGGAGTCA Rev, GGTCATTGATGGCAACAA | 99 |
ChIP-qPCR | ||
Vegf promoter of C/EBPβ binding region | For, ATCCTACCCGGAGTTGGTG Rev, ACTAAGGCCAGTGTGCCAAT | 125 |
Vegf promoter of HIF1α binding region -1 | For, GAACAAGGGCTTCTGTCTGC Rev, GGAAGCCGAGCAGTTAGTCA | 120 |
Vegf promoter of HIF1α binding region -2 | For, TACTTGCCTTCCACGTAGCC Rev, CCCAACAGTTGCTTGTTTGA | 80 |
Vegf promoter of HIF1α binding region -3 | For, GAGTCTGCGTGAGGAAGGAC Rev, CCTGGTCTTCTCCCCTACCT | 102 |
Luciferase assay | ||
−1171/+115 | For, GGTACCGAGGGAGCCTTACCTCTACTCC Rev, AAGCTTGGCTGATGAGTCCGTTGAAT | 1286 |
−976/+115 (ΔC/EBPβ) | For, GGTACCGAACAAGGGCTTCTGTCTGC Rev, AAGCTTGGCTGATGAGTCCGTTGAAT | 1091 |
−1171/+115 (Mutation of HREs)-1 | For, GCATACTCTGGCTTCCACAGGTCGTCTCCCTCCG Rev, GAAGCCAGAGTATGCACTGTGGAGTCTGGCAGAG | 1286 |
−1171/+115 (Mutation of HREs) -2 | For, GCCTTCAGAGTAGCCCCCCGCCCCATA Rev, GGCTACTCTGAAGGCAAGTATGCTTAT | 1286 |
−1171/+115 (Mutation of HREs)-3,4 | For, GTGCTCTCTCTCATGTGCGTGTGTGTCTGGGTATAGTGTG Rev, CATGAGAGAGAGCACTCACATTGACACACGCGTCCTTCC | 1286 |
