Figure 7
From: PrimerROC: accurate condition-independent dimer prediction using ROC analysis
ROC analyses showing performance of dimer algorithms and proportion of correctly identified dimer-free primers. All ΔGs were calculated with standard 1 M Na+, except MFEprimer where the PCR conditions of 50 mM Na+ and 4.5 mM Mg2+ were used. The area under the curve (AUC) has a maximum value of 1 (yellow), with 0.5 being a prediction accuracy no better than chance (blue). The true negative rate (TNR) is at the dimer-free discrimination threshold and has a maximum value of 1 (yellow) and minimum value of 0 (blue). PrimerROC out-performs all other algorithms in every primer set—both in AUC and proportion of correctly classified primers at the dimer-free discrimination threshold. df = dimer free, pd = primer dimer, ab = ambiguous.
