Figure 8
From: PrimerROC: accurate condition-independent dimer prediction using ROC analysis
ROC analyses showing dimer prediction performance under altered PCR conditions in 14 bp fusion set. All ΔGs were calculated with standard 1 M Na+, except MFEprimer where the PCR conditions of 50 mM Na+ and 4.5 mM Mg2+ were used. The area under the curve (AUC) has a maximum value of 1 (yellow), with 0.5 being a prediction accuracy no better than chance (blue). The true negative rate (TNR) is at the dimer-free discrimination threshold and has a maximum value of 1 (yellow) and minimum value of 0 (blue). PrimerROC out-performs all other algorithms under each condition tested—both in AUC and proportion of correctly classified primers at the dimer-free discrimination threshold. The predictive performance of PimerROC is also more robust than the other tools under the varying conditions tested. df = dimer free, pd = primer dimer (using stringent dimer classification). Conditions marked with an asterisk (*) contain 0.5X CES. These analyses were performed on the first 48 primers pairs of the 14 base pair fusion set.
