Figure 9
From: PrimerROC: accurate condition-independent dimer prediction using ROC analysis
ROC analyses showing dimer prediction of in-house ΔG calculated with varying temperatures and salt concentrations. The area under the curve (AUC) has a maximum value of 1 (yellow = 0.87), with 0.5 being a prediction accuracy no better than chance (blue = 0.69). The true negative rate (TNR) is at the dimer-free discrimination threshold and has a maximum value of 1 (yellow = 0.5) and minimum value of 0 (blue). Colours correspond to the maximum and minimum values within this set, allowing for better visualization of differences between conditions. Altering temperature and sodium concentration parameters when calculating ΔG can greatly impact its predictive accuracy. However, the standard 37 °C with 1 M sodium approaches maximum accuracy, with a lower sodium concentration of 0.5 M giving slightly better performance. PrimerROC results in substantially greater prediction accuracy over ΔG alone (AUC = 0.94 and TNR = 0.56). Analyses were performed on the combined set of all fusion primers using stringent dimer classification.
