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Figure 1

From: Structural features distinguishing infectious ex vivo mammalian prions from non-infectious fibrillar assemblies generated in vitro

Figure 1

Non-infectious recombinant PrP fibrils imaged by negative stain EM, cryo-EM and AFM. (a) EM image of fibrils stained with NanoW at pH 6.8. (b) Cryo-EM image of unstained fibrils in vitreous ice. The inset shows a magnified image of one fibril. Protein-A gold 10 nm were added to this sample as fiducial markers. (c) 1 µm × 1 µm scan showing height images obtained by AFM. (d) An enlarged image of one fibril from panel c with inset showing repeating structure along the fibril length (denoted by arrows) at 7.1 ± 0.8 nm intervals. Scale bars, 100 nm main panels a, b and c, 10 nm, main panel d and inset panel b, 5 nm, inset panel d.

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