Table 2 Adhesion force interaction measurements of RML prion rods and recombinant PrP fibrils using AFM.

From: Structural features distinguishing infectious ex vivo mammalian prions from non-infectious fibrillar assemblies generated in vitro

Sample

Adhesion Force Interaction (nN) Mean ± SD

Mica Surface

Fibre surface

Central gap

RML prion rods

0.69 ± 0.07

(n = 90)

0.49 ± 0.11

(n = 8 rods; 242 total measurements)*,†

0.87 ± 0.08

(n = 8 rods; 105 total measurements)*,†

Recombinant PrP fibrils

0.82 ± 0.11

(n = 160)

0.54 ± 0.16

(n = 6 fibrils; 183 total measurements)*

N/A

  1. *Means were generated from multiple measurements (>10) made along the length of each individual object (8 RML PrP rods or 6 recombinant PrP fibrils).
  2. †Difference in the mean values between the fibre surface and the central gap of the RML rods is statistically significant (p < 0.0001, unpaired t test with Welch correction).
  3. N/A, not applicable.
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