Figure 5
Low-pH vesicles within etching filopodia located at the bottom of an excavated pit viewed from the top. (a) Transmitted light image of etching filopodia indicated by black arrows and corresponding SEM image (top left) of excavated chip pits without etching filopodia (dashed square). (b) Z-stack imaging of the same area, where red fluorescence represents low pH, showing numerous vesicles with low pH following the outline of etching filopodia. (c) Processed image with pH quantification showing lower pH within vesicles with detail enlarged in (d).
