Figure 3
Disruption of only Tin2 of the shelterin core complex after 3 wk of silica inhalation. Inactivation of (A) POT1 and (C) Tin2 expression in lung cDNA at 16 hr after a 3-wk silica inhalation exposure (15 mg/m3, 6 h/d, 5 d/wk, 3 wk). (B) TPP1 (D) TRF1 (E) TRF2 and (F) RAP1 remained unchanged. All shelterin components expression (G) POT1 (H) TPP1 (I) Tin2 (J) TRF1 (K) TRF2 except (L) RAP1 were significantly downregulated in lung cDNA at 16 hr after a 6-wk silica inhalation exposure (15 mg/m3, 6 h/d, 5 d/wk, 6 wk). All shelterin components expression (M) POT1 (N) TPP1 (O) Tin2 (P) TRF1 (Q) TRF2 and (R) RAP1 were significantly downregulated in lung cDNA at 16 hr after a 12-wk silica inhalation exposure (15 mg/m3, 6 h/d, 5 d/wk, 12 wk). Rats exposed to filtered air served as the controls; n = 8; values are means ± standard error; *significantly different from corresponding air control, p < 0.05.
