Figure 1
From: ENOblock inhibits the pathology of diet-induced obesity
ENOblock effect on the induction of adipogenic gene expression in preadipocytes. (A) Schematic of the compound treatment protocol in primary WAT preadipocytes. (B) Effect of 72 h treatment with 10 µM forsoklin, 1 µM rapamycin or 10 µM ENOblock on the expression of adipogenesis regulatory genes. (C) Expression of oxidative phosphorylation regulatory genes after compound treatment. (D) Expression of thermogenesis regulatory genes after compound treatment. (E) Schematic of the compound pre-treatment protocol in WAT preadipocytes undergoing adipogenic differentiation. (F) Effect of treatment with 10 µM forsoklin, 1 µM rapamycin or 10 µM ENOblock on the expression of adipogenesis regulatory genes in differentiating preadipocytes. (G) Expression of oxidative phosphorylation regulatory genes after compound treatment. (H) Expression of thermogenesis regulatory genes after compound treatment. The treatment concentrations of forskolin, rapamycin or ENOblock were based on the following references7,87,88. n = 9; ns: not significantly different. *, ** or ***: significantly different from the corresponding ‘Control’ or ‘Untreated’ respectively with p < 0.05, p < 0.01 or p < 0.001; ## or ###: significantly different from the corresponding ‘ENOblock’, ж, жж or жжж: significantly different from the corresponding ‘Forskolin’.
