Figure 4

PFV intasome autointegration activity. PFV intasomes with Cy5 labeled viral donor DNA were incubated for variable time at 37 °C in the absence or presence of 5 mM PCA. (a) Reaction products were separated by agarose gel electrophoresis and imaged for Cy5 fluorescence. (b) Fluorescent products were analyzed as a fraction of total fluorescent signal in each lane. There appears to be similar AI product accumulation regardless of PCA addition.