Figure 5
Rescue of PFV intasome activity by additives present during preincubation. PFV FL intasomes were incubated at 37 °C for 5 min. Following the preincubation, target DNA was added and reactions were incubated for an additional 5 min at 37 °C. Additives included in the reactions were: 5 mM PCA (PCA), 0.1 mg/ml acetylated BSA (BSA), 5% DMSO (DMSO), 10% glycerol (Gly), 10% sucrose (Suc), and 10% PEG 6000 (PEG). Control reactions include no preincubation (No preinc) or no addition of an additive (−). Fluorescent products were analyzed and expressed as the fraction of fluorescent signal in each lane (bottom graph). Experiments were performed at least three times with at least two independent intasome preparations. Error bars indicate standard deviation.
