Figure 1

Prickle1^Bj/Bj mutants are microcephalic and have defects in the neural crest-derived skull. (a–c,e–g) Macroscopic views of Prickle1^+/+ (a–c) and Prickle1^Bj/Bj (e–g) littermates. (d) Schematic of the superior view of the skull vault. (h) quantification of skull vault measurements. (a,b,e,f) The Prickle1^Bj/Bj mutant heads are shorter proximal-distally when observed laterally from the external (e) and alizarin red and alcian blue stained specimens (f). (c,g) Superior (birds-eye) view of the skull vault demonstrates that the interfrontal suture (yellow lines). (d) Schematic of the skull vault showing the tissue of origin and below is the schema for the measurements in (h). (h) The proximal-distal shortening of the Prickle1^Bj/Bj mutants is most profound in the nasal region. c, coronal suture; f, frontal bone; if, interfrontal suture; ip, interparietal bone; m, mesoderm; n, nasal bone; ncc, neural-crest cell; p, parietal bone; s, sagittal suture. Scale bar = 0.5 cm.