Figure 3
From: Prickle1 regulates differentiation of frontal bone osteoblasts
Prickle1 protein is expressed in the craniofacial mesenchyme. We performed immunohistochemistry to Prickle1 protein at E12.5 (a,b) and E15.5 (d–f) and detected Prickle1 mRNA expression via Q-PCR at E15.5 (g). (a) Prickle1 protein is found in the frontal bone condensation at E12.5 (black outline). (b) A subset of the cells in the frontal bone express Prickle1 protein at E12.5 (black outline). (c) A horizontal section through the E15.5 skull showing von Kossa stained mineralized tissue in the frontal and parietal bones. (d) Section adjacent to c, stained with immunohistochemistry to Prickle1. (e) The E15.5 frontal bone expresses Prickle1 protein. (f) The E15.5 parietal bone also expresses Prickle1 protein. (e) At E15.5, Prickle1 mRNA expression is equivalent between the frontal and parietal bones (n = 7). Scale bars: a = 100 μm; b-f = 200 μm. cs, coronal suture; fb, frontal bone; pb, parietal bone.
