Figure 4

Mineralization defects in the Prickle1^Bj/Bj mutants. Histological analysis with von Kossa staining of Prickle1^+/+ (a–c) and Prickle1^Bj/Bj (d–f) littermates at E15.5 (a,d) and E18.5 (b,c,e,f). (a,c) The smaller size of the Prickle1^Bj/Bj frontal bones is apparent at E15.5 and the osteogenic fronts do not extend as far apically as the littermate control (black arrow). (b,e) The interfrontal suture is enlarged between the Prickle1^Bj/Bj frontal bones (arrows). (c,f) The secondary palate is cleft and the maxillary and vomer (arrow) bones are displaced laterally. if, interfrontal suture; fb, frontal bone; mxb, maxillary bone; ns, nasal septum; t, tongue; v, vomer. Scale bar = 100 μm and applies to all.