Figure 5
From: Prickle1 regulates differentiation of frontal bone osteoblasts
No change in the rate of proliferation or apoptosis in the E12.5 Prickle1Bj/Bj frontal bones. E12.5 Prickle1+/+ (a–d) and Prickle1Bj/Bj (f–i) littermates assayed for histology (haemotoxylin and eosin) staining (a,f), TUNEL staining (b,g), proliferation with BrdU immunofluorescence (c,h) and mitosis with phospho-histone H3 immunohistochemistry (d,i). (a,d) The frontal bone mesenchymal condensation (black outline) is present in both wildtype (a) and Prickle1Bj/Bj (f) littermates. (b,g) TUNEL-positive cells were found near the eye (arrowheads) and absent in the frontal bone primordia. (c,h) BrdU-positive cells (green) are found in the frontal bone primordium (white outline) of wildtype (c) and Prickle1Bj/Bj (h) littermates. (d,i) Few positive PHH3-positive cells (brown) are found in the frontal bone primordium of wildtype (d) and Prickle1Bj/Bj (i) littermates. (e) No difference in the ratio of BrdU-positive cells in the frontal bone primordia (n = 3). (j) No difference in the number of PHH3-positive cells in the frontal bones between genotypes (n = 3). Scale bar = 100 μm.
