Figure 7

Wnt/β-catenin and Hedgehog signaling is decreased in the E12.5 frontal bone primordium. Immunofluorescence of active β-catenin (a,e) and DIG-labelled section in situ hybridization to Lef1 (b,f), Patched1 (Ptch1) (c, g) and Gli1 (d,h) in E12.5 Prickle1^+/+ (a–d) and Prickle1^Bj/Bj (e–h) littermates. (a,e) The level of active β-catenin (ABC) protein is decreased in the frontal bone primordium, but is maintained in the surface epithelium in the Prickle1^Bj/Bj mutants compared with wild-type littermates. (b,f) Lef1 expression is reduced in the Prickle1^Bj/Bj frontal bone primordium and throughout the mesenchyme surrounding it compared with wild-type littermates. (c,g) Ptch1 expression is reduced in the Prickle1^Bj/Bj frontal bone primordium and throughout the mesenchyme surrounding it compared with control littermates. (d,h) Gli1 expression is also reduced in the Prickle1^Bj/Bj frontal bone primordium compared with control littermates. Scale bar = 100 μm.