Figure 8

Osteoblast migration is decreased in the frontal bone primordium. DIG-labeled section in situ hybridization to Twist1, Msx1, Msx2 and Engrailed1 (En1) to E12.5 Prickle1^+/+ (a–d) and Prickle1^Bj/Bj (e–h) coronal sections. (a,e) Decreased expression of Twist1 in the Prickle1^Bj/Bj compared with wild-type. (b,f) The expression of Msx1 is decreased in the mutant compared with wild-type. (c,g) The expression of Msx2 is slightly decreased in the Prickle1^Bj/Bj compared with wild-type. (d,h) The expression of En1 is similar in the Prickle1^Bj/Bj compared with wild type. Scale bar in a = 200 μm, and applies to all.