Table 1 Source-sensitivity and source-specificity results of the BacR HDA-strip assay and the BacR qPCR assay.

From: Detection of a microbial source tracking marker by isothermal helicase-dependent amplification and a nucleic acid lateral-flow strip test

Ruminants

Non-Ruminants

Source

Scientific name

HDA- strip

qPCRa

Source

Scientific name

HDA-strip

qPCR

Cattle 1

Bos Taurus

3/3

3/3

Human 1

Homo sapiens

0/3

0/3

Cattle 2

Bos Taurus

3/3

3/3

Human 2

Homo sapiens

0/3

0/3

Cattle 3

Bos Taurus

3/3

3/3

Human 3

Homo sapiens

0/3

0/3

Cattle 4

Bos Taurus

3/3

3/3

Human 4

Homo sapiens

0/3

0/3

Red sheep 1

Ovis aries

3/3

3/3

Pig

Sus scrofa domesticus

0/3

0/3

Red sheep 2

Ovis aries

3/3

3/3

Wild Boar

Sus scrofa

1/3

0/3

Red sheep 3

Ovis aries

3/3

3/3

Horse

Equus caballus

0/3

0/3

Red sheep 4

Ovis aries

3/3

3/3

Horse

Equus caballus

0/3

0/3

Goat 1

Capra hircus

3/3

3/3

Chicken

Gallus gallus

0/3

0/3

Goat 2

Capra hircus

3/3

3/3

Red Fox

Vupes vulpes

0/3

0/3

Red deer 1

Cervus elaphus

3/3

3/3

Dog

Canis familiaris

0/3

0/3

Red deer 2

Cervus elaphus

3/3

3/3

Cat

Felis catus

0/3

0/3

Red deer 3

Cervus elaphus

3/3

3/3

Beaver

Castor fibre

0/3

0/3

Red deer 4

Cervus elaphus

3/3

3/3

Common carp

Cyprinus carpio

0/3

0/3

Roe deer 1

Capreolus capreolus

3/3

3/3

Zander

Sander luciperca

0/3

0/3

Roe deer 2

Capreolus capreolus

3/3

3/3

Common bream

Abramis brama

0/3

0/3

Chamois 1

Rupicapra rupicapra

3/3

3/3

Brook trout

Salvelinus fontinalis

0/3

0/3

Chamois 2

Rupicapra rupicapra

3/3

3/3

Greylag goose

Anser anser

0/3

0/3

Ibex 1

Capra ibex

3/3

3/3

Grey heron

Ardea cinerea

0/3

0/3

Ibex 2

Capra ibex

3/3

3/3

Duck

Anas platyrhynchos

0/3

0/3

Source-sensitivityb

100%

100%

Source-specificityc

98%

100%

  1. Assays were evaluated on 20 single ruminant (target) and 20 single non-ruminant (non-target) faecal samples, each tested at a concentration of 1 ng extracted DNA per reaction. The results are given as the number of positive reactions from triplicate analyses and as calculated sensitivity and specificity percentages.
  2. aMarker copy numbers ranged from 104 to 105 copies per reaction based on qPCR analysis with plasmid standards.
  3. bSource sensitivity (%): (true positives)/(true positives + false negatives) × 100.
  4. cSource specificity (%): (true negatives)/(true negatives + false positives) × 100.
Back to article page