Table 3 Oligonucleotides used in this study.

From: Detection of a microbial source tracking marker by isothermal helicase-dependent amplification and a nucleic acid lateral-flow strip test

Name

Function

Sequence (5′–3′)a

Reference

Binding positions b

H-BacR_f

HDA forward primer

GTATCCAACCTTCCCGTTAC

This study

91–110

H-BacR_r

HDA reverse primer

ATCCCCATCCGTTACCG

This study

189–205

H-BacR_CP

Strip test capture probe

AAAGGGAGATT(A)20-BtnTg

This study

120–127

H-BacR_DP

Strip test detector probe

ThiC6-(A)20GCCTTCCG

This study

128–138

H-BacR_CL

Strip test control probe

Btn-(A)20GAAGGCTTTTT

This study

—

BacR_f

qPCR forward primer

GCGTATCCAACCTTCCCG

27

89–106

BacR_r

qPCR reverse primer

CATCCCCATCCGTTACCG

27

189–206

BacR_p

qPCR probe

FAM-CTTCCGAAAGGGAGATT-NFQ-MGB

27

122–138

  1. aAbbreviations: FAM, 6-carboxyfluorescein; NFQ, non-fluorescent quencher; MGB, minor groove binder; Btn/BtnTg, Biotin; ThiC6, Thiol group. (A)20 is a sequence of 20 oligo(dA) used as a spacer. bBinding positions refer to GenBank accession number AF233400.
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