Table 1 Arrestin-mCherry fusion constructs.
From: Arrestin-1 engineering facilitates complex stabilization with native rhodopsin
Mutants | Binding capabilitya | Functional expression level relative to WT | Activity level relative to WT |
|---|---|---|---|
WT | 100% | 100% (s.d.: N/A, n: 74) | 100% (s.d.: 16, n: 5) |
D303A, T304A, E341A, F375A | 710% | 101% (s.d.: 20, n: 4) | 44% (s.d.: 16, n: 5) |
R171A, T304A, E341A, F375A | 720% | 127% (s.d.: 12, n: 4) | 88% (s.d.: 27, n: 8) |
T304A, E341A, F375A | 671% | 68% (s.d.: 16, n: 3) | 149% (s.d.: 55, n: 4) |
T304A, F375A | 502% | 86% (s.d.: 8, n: 3) | 106% (s.d.: 31, n: 4) |
F375A | 322% | 106% (s.d.: 26, n: 17) | 102% (s.d.: 20, n: 4) |
